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β-Hydroxybutyrate (β-HB) has a molecular weight of 104.1 kD and accounts for about 70% of total ketone bodies. In patients with severe acidosis, the increased use of NADH in the body due to acidosis promotes the formation of β-HB from acetoacetate, raising the β-HB to acetoacetate ratio from the normal 2:1 to 16:1. The enzymatic method for measuring β-HB is the most sensitive, rapid, economical, and simple, making it a commonly used clinical method.
0.031-0.263 mmol/L. < 0.7 mmol/L. (Colorimetric method)
The measurement of serum β-HB is mainly used for the differential diagnosis of ketoacidosis and for monitoring such patients.
Separate the blood sample within 24 hours after collection, and store the sample at 4°C for no more than 1 week.
Severely hemolyzed samples (hemoglobin up to 110 g/L) can reduce the β-HB concentration by 0.8 mmol/L.
Severe jaundice samples with bilirubin > 273 µmol/L can significantly reduce β-HB values.