Cell Culture Dilution Calculator

Dilution Formula:

\[ V2 = V1 \times \frac{C1}{C2} \]

Final Volume (V2):

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1. What is Cell Culture Dilution?

Cell culture dilution is the process of adjusting the concentration of cells in a culture medium to achieve a desired cell density for experiments or subculturing. Proper dilution is essential for maintaining healthy cell growth and consistent experimental conditions.

2. How Does the Calculator Work?

The calculator uses the dilution formula:

\[ V2 = V1 \times \frac{C1}{C2} \]

Where:

\( V2 \) — Final volume after dilution (mL)
\( V1 \) — Initial volume before dilution (mL)
\( C1 \) — Initial cell concentration (cells/mL)
\( C2 \) — Desired final cell concentration (cells/mL)

Explanation: The formula calculates the total volume needed to dilute an initial cell suspension to achieve the desired concentration.

3. Importance of Proper Dilution

Details: Accurate cell dilution is crucial for maintaining consistent cell densities in experiments, preventing overcrowding, and ensuring proper nutrient availability for cell growth.

4. Using the Calculator

Tips: Enter the initial volume in mL, initial cell concentration in cells/mL, and desired final concentration in cells/mL. All values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: How do I count cells for accurate dilution?
A: Use a hemocytometer or automated cell counter after trypan blue staining to determine viable cell concentration.

Q2: What's the typical cell concentration for subculturing?
A: This varies by cell type, but common ranges are 1-5×10⁴ cells/mL for adherent cells and 2-5×10⁵ cells/mL for suspension cultures.

Q3: Should I account for cell doubling time?
A: For long-term cultures, yes. The calculated dilution provides the starting concentration; cells will continue to multiply.

Q4: How does this differ from serial dilution?
A: This calculates a single-step dilution. Serial dilution involves multiple sequential dilution steps for greater dilution factors.

Q5: What medium should I use for dilution?
A: Use the same culture medium that the cells are growing in, pre-warmed to appropriate temperature with required supplements.