1. What is WBC Count?

The WBC (White Blood Cell) count measures the number of white blood cells in a volume of blood. It's an important indicator of immune system function and helps diagnose infections, inflammation, and blood disorders.

2. How is WBC Count Calculated?

The calculator uses the hemocytometer formula:

Where:

• Cells Counted — Total WBCs counted in all squares
• Dilution Factor — Typically 20 for WBC counts (1:20 dilution)
• Number of Squares Counted — Usually 4 corner squares of hemocytometer
• 10^4 — Conversion factor for volume (0.1 μL to 1 μL) and area

Explanation: The formula accounts for the dilution of blood sample and the volume of blood examined in the hemocytometer chambers.

3. Importance of WBC Count

Details: WBC count helps diagnose infections, monitor immune disorders, assess bone marrow function, and track response to treatments like chemotherapy.

4. Using the Calculator

Tips: Enter the total cells counted, dilution factor (typically 20 for WBC counts), and number of squares counted (usually 4). All values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: What is a normal WBC count range?
A: Normal range is typically 4,000-11,000 cells/μL in adults, though ranges vary by age and laboratory.

Q2: Why is blood diluted before counting?
A: Blood is diluted to make counting feasible and to lyse red blood cells which would otherwise obscure WBCs.

Q3: How many squares should be counted?
A: Typically the four large corner squares are counted (total area of 4 mm²), but some protocols may differ.

Q4: What affects WBC count accuracy?
A: Proper mixing, dilution accuracy, counting technique, and sample quality all affect results.

Q5: When is manual counting preferred over automated?
A: Manual counting is used when automated counters are unavailable or when verifying abnormal automated results.