1. What is Dilution Factor in HPLC?

The Dilution Factor (DF) in HPLC is a dimensionless number that represents how much a sample has been diluted. It's the ratio of the total volume after dilution to the original sample volume.

2. How Does the Calculator Work?

The calculator uses the dilution factor formula:

Where:

• \( DF \) — Dilution Factor (unitless)
• \( V_{sample} \) — Original sample volume (mL)
• \( V_{diluent} \) — Volume of diluent added (mL)

Explanation: The formula calculates how many times the original sample has been diluted by comparing the final volume to the initial volume.

3. Importance of Dilution Factor

Details: The dilution factor is crucial in HPLC for:

• Calculating original concentrations from diluted samples
• Ensuring samples are within the detector's linear range
• Method development and validation
• Data interpretation and reporting

4. Using the Calculator

Tips:

• Enter sample volume in mL (must be > 0)
• Enter diluent volume in mL
• The calculator will compute the dilution factor
• For serial dilutions, multiply the DF of each step

5. Frequently Asked Questions (FAQ)

Q1: What if I have serial dilutions?
A: Multiply the dilution factors of each step. For example, two 10-fold dilutions give a total DF of 100.

Q2: How does DF relate to concentration?
A: Original concentration = Measured concentration × DF

Q3: What's the difference between DF and dilution ratio?
A: Dilution ratio is V_sample:V_diluent, while DF is the mathematical factor used in calculations.

Q4: Can DF be less than 1?
A: No, DF is always ≥1. A DF of 1 means no dilution occurred.

Q5: How precise should my volume measurements be?
A: Use volumetric glassware appropriate for your needed precision (e.g., pipettes for small volumes, flasks for larger volumes).