1. What is Protein Molarity?

Protein molarity is the concentration of a protein solution expressed in moles per liter (M). It's essential for many biochemical experiments where precise protein concentrations are required.

2. How Does the Calculator Work?

The calculator uses the molarity equation:

Where:

• \( M \) — Molarity (mol/L or M)
• \( C \) — Protein concentration (mg/mL)
• \( MW \) — Molecular weight (g/mol)

Explanation: The equation converts mass concentration (mg/mL) to molar concentration (mol/L) by accounting for the protein's molecular weight.

3. Importance of Protein Molarity

Details: Knowing the molar concentration of proteins is crucial for:

• Enzyme kinetics studies (Km, Vmax calculations)
• Binding assays
• Stoichiometric calculations
• Preparing solutions with specific molar ratios

4. Using the Calculator

Tips:

• Enter protein concentration in mg/mL
• Enter molecular weight in g/mol (Dalton)
• For multimeric proteins, use the molecular weight of the entire complex
• All values must be positive numbers

5. Frequently Asked Questions (FAQ)

Q1: How do I find my protein's molecular weight?
A: You can calculate it from the amino acid sequence or use published values. Many protein databases provide this information.

Q2: What if my protein has multiple subunits?
A: Use the total molecular weight of the complete complex (sum of all subunits).

Q3: Does this work for protein mixtures?
A: No, this calculator is for pure proteins. For mixtures, you would need to know the concentration of each component.

Q4: How accurate is this calculation?
A: The calculation is mathematically precise, but accuracy depends on your input values (especially the molecular weight).

Q5: Can I convert back from molarity to mg/mL?
A: Yes, by rearranging the equation: \( C = M \times MW \). Just multiply molarity by molecular weight.