1. What is the NEB DNA Molarity Equation?

The NEB DNA Molarity equation calculates the molar concentration of DNA from its mass concentration and molecular weight. This is particularly useful for molecular biology applications where precise molar concentrations are needed for reactions.

2. How Does the Calculator Work?

The calculator uses the DNA molarity equation:

Where:

• \( M \) — DNA molarity (pM)
• \( C \) — DNA concentration (ng/μL)
• \( MW \) — Molecular weight (g/mol)

Explanation: The equation converts mass concentration to molar concentration, with the 10^6 factor converting from moles/L to picomoles/μL.

3. Importance of DNA Molarity Calculation

Details: Accurate DNA molarity is essential for setting up restriction digests, ligations, PCR reactions, and other molecular biology techniques where stoichiometric ratios are important.

4. Using the Calculator

Tips: Enter DNA concentration in ng/μL and molecular weight in g/mol. For double-stranded DNA, MW can be calculated as (length in bp) × 650 g/mol/bp.

5. Frequently Asked Questions (FAQ)

Q1: Why calculate DNA molarity instead of using concentration?
A: Molarity allows for accurate stoichiometric calculations in reactions where molecules interact in molar ratios.

Q2: How do I determine molecular weight of my DNA?
A: For dsDNA, multiply length in base pairs by ~650 g/mol/bp. For ssDNA or RNA, use ~330 g/mol/nt.

Q3: What's the difference between pM and pmol/μL?
A: They are equivalent units (1 pM = 1 pmol/L = 0.001 pmol/μL).

Q4: Can I use this for oligonucleotides?
A: Yes, but be sure to use the correct molecular weight (typically provided by the manufacturer).

Q5: How accurate is this calculation?
A: The calculation is mathematically precise, but accuracy depends on your input measurements.