1. What is the Ng/μL to Fmol Conversion?

This calculator converts mass concentration (ng/μL) to molar concentration (fmol) using the molecular weight of the substance. This conversion is essential in molecular biology and biochemistry when preparing solutions with specific molar concentrations.

2. How Does the Calculator Work?

The calculator uses the conversion formula:

Where:

• \( ng \) — Nanograms of substance
• \( MW \) — Molecular weight in g/mol
• \( 10^6 \) — Conversion factor (ng to g and mol to fmol)

Explanation: The formula first converts ng to grams (1 ng = 10⁻⁹ g), then divides by molecular weight to get moles, and finally converts to femtomoles (1 fmol = 10⁻¹⁵ mol).

3. Importance of Molar Calculations

Details: Accurate molar concentration calculations are crucial for experiments requiring precise stoichiometry, such as PCR, enzyme reactions, and molecular cloning.

4. Using the Calculator

Tips: Enter the amount in nanograms and the molecular weight in g/mol. Ensure both values are positive numbers for accurate calculation.

5. Frequently Asked Questions (FAQ)

Q1: Why convert ng to fmol?
A: Many biological experiments require molar concentrations rather than mass concentrations for proper stoichiometric ratios.

Q2: Where can I find molecular weights?
A: Molecular weights can be found in chemical databases, product specifications, or calculated from chemical formulas.

Q3: Does this work for any substance?
A: Yes, as long as you know the molecular weight of the substance in question.

Q4: What if my substance is in solution?
A: For solutions, you'll need to know the concentration in ng/μL to use this calculator.

Q5: How precise is this conversion?
A: The precision depends on the accuracy of your input values. The calculator provides results to two decimal places.