1. What is ng/μL to μM Conversion?

The ng/μL to μM conversion calculates molar concentration from mass concentration using the molecular weight of the substance. This is essential in biochemistry and molecular biology when preparing solutions with specific molar concentrations.

2. How Does the Calculator Work?

The calculator uses the conversion formula:

Where:

• \( ng/\mu L \) — Mass concentration in nanograms per microliter
• \( MW \) — Molecular weight in grams per mole
• \( 10^3 \) — Conversion factor (1000 ng = 1 μg, and 1 μL = 10^-6 L)

Explanation: The formula converts mass per volume to moles per volume by dividing by the molecular weight and adjusting units appropriately.

3. Importance of Concentration Conversion

Details: Accurate concentration conversion is crucial for preparing solutions with precise molarities, which is essential for experiments, drug formulations, and biochemical assays.

4. Using the Calculator

Tips: Enter the concentration in ng/μL and the molecular weight in g/mol. Both values must be positive numbers. The result will be displayed in μM (micromolar).

5. Frequently Asked Questions (FAQ)

Q1: Why convert ng/μL to μM?
A: Many biological experiments require molar concentrations rather than mass concentrations, as reactions depend on the number of molecules present.

Q2: How accurate is this conversion?
A: The conversion is mathematically exact, but accuracy depends on the precision of your input values and the correct molecular weight.

Q3: Can I use this for proteins and nucleic acids?
A: Yes, as long as you know the molecular weight. For nucleic acids, use the average molecular weight of a base pair (660 g/mol for dsDNA).

Q4: What if my substance is in ng/mL?
A: First convert ng/mL to ng/μL by dividing by 1000, or use the formula: μM = (ng/mL) / MW.

Q5: How does temperature affect this conversion?
A: Temperature doesn't affect the conversion directly, but it may affect your actual measurements if volume changes with temperature.