1. What is Cell Dilution?

Cell dilution is a laboratory technique used to reduce the concentration of cells in a solution. The formula V₂ = V₁ × (C₁/C₂) calculates the final volume needed to achieve a desired concentration from an initial concentration.

2. How Does the Calculator Work?

The calculator uses the cell dilution equation:

Where:

• \( V_1 \) — Initial volume (mL)
• \( C_1 \) — Initial cell concentration (cells/mL)
• \( C_2 \) — Final desired cell concentration (cells/mL)
• \( V_2 \) — Final volume needed (mL)

Explanation: The equation maintains the same number of cells while changing the volume to achieve the desired concentration.

3. Importance of Cell Dilution

Details: Accurate cell dilution is crucial for experiments requiring specific cell concentrations, such as cell culture work, flow cytometry, and microbiology studies.

4. Using the Calculator

Tips: Enter initial volume in mL, initial and final concentrations in cells/mL. All values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: What if I know V₂ and need to find C₂?
A: Rearrange the formula: C₂ = (V₁ × C₁) / V₂

Q2: How precise should my measurements be?
A: For most applications, measuring to 2 decimal places is sufficient, but follow your lab's specific protocols.

Q3: Does this work for non-cell particles?
A: Yes, the formula works for any particles in suspension (bacteria, beads, etc.) as long as concentration units match.

Q4: What if my final volume is too large?
A: You may need to perform serial dilutions - dilute in multiple steps to achieve very high dilution factors.

Q5: How do I account for dilution errors?
A: Always mix thoroughly after dilution and consider verifying concentration with a hemocytometer or automated cell counter.