Pmol to Mass Calculator Bioline Nucleic Acids

Mass Calculation Formula:

\[ \text{mass (ng)} = \frac{\text{pmol} \times \text{MW (g/mol)}}{1000} \]

Mass:

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1. What is the Pmol to Mass Calculation?

The pmol to mass calculation converts the amount of nucleic acid in picomoles (pmol) to mass in nanograms (ng) using the molecular weight of the oligo. This is essential for accurate preparation of nucleic acid solutions in molecular biology experiments.

2. How Does the Calculator Work?

The calculator uses the following formula:

\[ \text{mass (ng)} = \frac{\text{pmol} \times \text{MW (g/mol)}}{1000} \]

Where:

\( pmol \) — Amount of oligo in picomoles
\( MW \) — Molecular weight of the oligo in g/mol
1000 — Conversion factor (1000 pmol = 1 nmol; 1000 ng = 1 μg)

Explanation: The formula accounts for the direct relationship between the amount of substance (pmol) and its mass, scaled by the molecular weight of the specific nucleic acid.

3. Importance of Mass Calculation

Details: Accurate mass calculation is crucial for preparing solutions with precise concentrations, ensuring reproducibility in experiments like PCR, sequencing, and other molecular biology applications.

4. Using the Calculator

Tips: Enter the amount of oligo in pmol and the molecular weight in g/mol. Both values must be positive numbers. The molecular weight can be calculated using online tools or provided by the oligo manufacturer.

5. Frequently Asked Questions (FAQ)

Q1: How do I determine the molecular weight of my oligo?
A: Molecular weight can be calculated based on the sequence using online tools or provided by the manufacturer (like Bioline). For DNA, the average MW of a nucleotide is ~330 g/mol.

Q2: Why is the result in nanograms?
A: Nanograms are a convenient unit for typical amounts of nucleic acids used in molecular biology protocols. 1 ng/μL is a common working concentration.

Q3: Can this be used for both DNA and RNA?
A: Yes, as long as you use the correct molecular weight for your specific nucleic acid (RNA nucleotides have slightly higher MW than DNA).

Q4: How accurate is this calculation?
A: The calculation is mathematically precise. Accuracy depends on the precision of your pmol measurement and the correctness of the MW value used.

Q5: What if my oligo is modified?
A: For modified oligos (e.g., with fluorescent labels), you must include the modification's contribution to the total molecular weight.