Pmol to Mass Calculator Bioline

Mass Calculation Formula:

\[ \text{mass (ng)} = \frac{\text{pmol} \times \text{MW (g/mol)}}{1000} \]

Mass:

Unit Converter ▲

Unit Converter ▼

From:	To:

1. What is the Pmol to Mass Conversion?

The pmol to mass conversion calculates the mass of a substance in nanograms (ng) from its amount in picomoles (pmol) and its molecular weight (MW). This is particularly useful in molecular biology and biochemistry for preparing solutions with precise concentrations.

2. How Does the Calculator Work?

The calculator uses the following formula:

\[ \text{mass (ng)} = \frac{\text{pmol} \times \text{MW (g/mol)}}{1000} \]

Where:

pmol — Amount in picomoles (10^-12 moles)
MW — Molecular weight in grams per mole (g/mol)
1000 — Conversion factor (1 ng = 10^-9 g)

Explanation: The formula converts the molar amount (pmol) to mass (ng) using the substance's molecular weight, with appropriate unit conversions.

3. Importance of Mass Calculation

Details: Accurate mass calculation is essential for preparing solutions with precise concentrations, especially in molecular biology experiments, PCR reactions, and other applications where exact amounts of DNA, RNA, or proteins are required.

4. Using the Calculator

Tips: Enter the amount in pmol and the molecular weight in g/mol. Both values must be positive numbers. The result will be displayed in nanograms (ng).

5. Frequently Asked Questions (FAQ)

Q1: Why is the result in nanograms?
A: Nanograms are a convenient unit for the small quantities typically used in molecular biology. 1 ng = 10^-9 grams.

Q2: How do I find the molecular weight of my substance?
A: For oligonucleotides, use the formula provided by the manufacturer. For proteins, calculate from the amino acid sequence or use published values.

Q3: Can I use this for double-stranded DNA?
A: Yes, but remember to use the molecular weight of the double-stranded molecule (average MW of a base pair is ~660 g/mol).

Q4: What's the difference between pmol and ng?
A: pmol is a unit of amount (number of molecules), while ng is a unit of mass. The conversion depends on the molecular weight of the specific substance.

Q5: How accurate is this calculation?
A: The calculation is mathematically precise. Accuracy depends on the precision of your pmol measurement and the accuracy of the molecular weight value.