RNA Molecular Weight Formula:
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RNA molecular weight is the sum of the atomic weights of all atoms in an RNA molecule. It's calculated by adding up the molecular weights of all nucleotide bases in the RNA sequence.
The calculator uses the formula:
Where:
Explanation: The calculator sums the products of each base count multiplied by its respective molecular weight.
Details: Knowing the molecular weight of RNA is essential for laboratory techniques like gel electrophoresis, PCR, and RNA quantification. It helps in determining appropriate concentrations for experiments.
Tips: Enter the count of each RNA base (A, U, G, C). The calculator will sum their individual molecular weights to give the total molecular weight in g/mol.
Q1: Does this include the phosphate backbone?
A: Yes, the molecular weights used include the complete nucleotide (base + sugar + phosphate).
Q2: How accurate is this calculation?
A: This provides an estimate. Actual molecular weight may vary slightly due to isotopic distributions and modifications.
Q3: Can I calculate for modified bases?
A: This calculator only handles standard bases. Modified bases require separate molecular weight values.
Q4: What about water molecules lost during polymerization?
A: The calculation accounts for water loss during phosphodiester bond formation.
Q5: Can this be used for single-stranded and double-stranded RNA?
A: This calculates single-stranded RNA. For dsRNA, multiply by 2 and subtract water loss from base pairing.