SSDNA Molecular Weight Calculator

SSDNA Molecular Weight Formula:

\[ MW = (A \times 313.2) + (T \times 304.2) + (C \times 289.2) + (G \times 329.2) - 61.0 \]

Molecular Weight:

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1. What is SSDNA Molecular Weight?

The molecular weight of single-stranded DNA (ssDNA) is calculated based on the count of each nucleotide (A, T, C, G) and their respective molecular weights, minus the molecular weight of water for each phosphodiester bond.

2. How Does the Calculator Work?

The calculator uses the ssDNA molecular weight formula:

\[ MW = (A \times 313.2) + (T \times 304.2) + (C \times 289.2) + (G \times 329.2) - 61.0 \]

Where:

\( A \) — Count of Adenine bases
\( T \) — Count of Thymine bases
\( C \) — Count of Cytosine bases
\( G \) — Count of Guanine bases
313.2 — Molecular weight of Adenine (g/mol)
304.2 — Molecular weight of Thymine (g/mol)
289.2 — Molecular weight of Cytosine (g/mol)
329.2 — Molecular weight of Guanine (g/mol)
61.0 — Adjustment for water loss in phosphodiester bonds

3. Importance of Molecular Weight Calculation

Details: Knowing the molecular weight of ssDNA is essential for various molecular biology applications including PCR, gel electrophoresis, DNA sequencing, and quantitative measurements in experiments.

4. Using the Calculator

Tips: Enter the count of each nucleotide (A, T, C, G) in your ssDNA sequence. All values must be non-negative integers. The calculator will compute the total molecular weight in g/mol.

5. Frequently Asked Questions (FAQ)

Q1: Why are the molecular weights different for each base?
A: Each nucleotide has a unique molecular structure with different numbers and types of atoms, resulting in different molecular weights.

Q2: Does this calculator work for RNA?
A: No, this is for ssDNA only. RNA uses uracil (U) instead of thymine (T) and has slightly different molecular weights.

Q3: What about modified nucleotides?
A: This calculator uses standard nucleotide weights. Modified nucleotides would require adjustment of the molecular weights.

Q4: Why subtract 61.0?
A: This accounts for the loss of water (H₂O, MW=18) during phosphodiester bond formation between nucleotides, plus an additional proton.

Q5: How accurate is this calculation?
A: It provides a theoretical molecular weight based on average isotopic composition. For precise measurements, mass spectrometry would be needed.