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Tocris Dilution Formula:
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The Tocris dilution formula calculates the molar concentration of a solution when you know the mass of solute, its molecular weight, and the total volume of solution. This is essential for preparing accurate reagent solutions in laboratory settings.
The calculator uses the Tocris dilution formula:
Where:
Explanation: The formula converts mass concentration to molar concentration by accounting for the molecular weight of the compound and the total solution volume.
Details: Precise solution preparation is critical for experimental reproducibility, accurate dosing in biological assays, and ensuring consistent results in research applications.
Tips: Enter mass in grams, molecular weight in g/mol, and volume in liters. All values must be positive numbers. For best results, use analytical balance measurements and high-precision volumetric glassware.
Q1: Why is molecular weight important in dilution calculations?
A: Molecular weight converts between mass (grams) and moles, allowing you to calculate molar concentration which is the standard unit for chemical solutions.
Q2: What if my volume is in mL instead of liters?
A: Convert mL to L by dividing by 1000 (since 1 L = 1000 mL) before entering the value, or modify the calculator to accept mL input.
Q3: How precise should my measurements be?
A: For research-grade solutions, measure mass to at least 4 decimal places (0.0001 g) and use volumetric flasks for accurate volume measurements.
Q4: Does this work for any chemical compound?
A: Yes, as long as you know the exact molecular weight of the compound. For mixtures or compounds with variable composition, additional calculations are needed.
Q5: How do I prepare a dilution series from a stock solution?
A: First calculate your stock concentration using this calculator, then use C1V1 = C2V2 to calculate dilution volumes for your desired concentrations.